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KMID : 0363019740040010025
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Journal of Korean Academy of Periodontology
1974 Volume.4 No. 1 p.25 ~ p.34
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Immunopathological Studies on the Inflamed Gingival Tissues-Localization of Human r-Globulin By Fluorescent Antibody Technique-
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Choi Sang-Mook
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Abstract
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Recently it has been suggested that the antigenic factor by oral bacteria resident in the gingival pocket is responsible for the hypersensitivity which causes periodontal diseases. The present research .attempts to prove the localization of immunoglobulins in the human inflamed gin giva-by a fluotescefice antibody technique. Out of cases the human inflamed gingivae were excised from periodontal patients and´ applied fluorescent antibody technique for the detection of the localization of immunoglobulins. The tissue obtained were immediately put into 95% cold ethanol and passed through cold absolute alcohol and xylene. The principal technique employed in these studies was Sainte-Marie´s method. Paraffin embedding and sectioning were also performed with the same method. The results are as follows: 1. There were no significant relationships of the localization of, immunoglobulins in epithelial layer between normal and inflamed gingiva-but in connective tissues in there were relationship between gingival inflammation and the localization of, immunoglobulins. 2. The distribution of intense specific fluoreseence of immunoglobdlin in epithelial layer was classified as 3 types a. localized in basal cell layer b. all of ´the epithelial -layer especially in intercellular spaces c. at randomly in prickle cell layer 4. The6 small particles-like or granular specific fluorescence was observed in necrotic area. 5. Intense specific fluorescence was observed in capillary walls. 6. Specific-Human immunoglobulin was observed in cytoplasm fluorescence Of immunoglobulin of small round cells, especially in plasma cells rather than in lymphocytes. 7. Russel´s body was revealed positive reactions.
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